Human MutL-complexes monitor homologous recombination independently of mismatch repair
نویسندگان
چکیده
منابع مشابه
Mismatch repair during homologous and homeologous recombination.
Homologous recombination (HR) and mismatch repair (MMR) are inextricably linked. HR pairs homologous chromosomes before meiosis I and is ultimately responsible for generating genetic diversity during sexual reproduction. HR is initiated in meiosis by numerous programmed DNA double-strand breaks (DSBs; several hundred in mammals). A characteristic feature of HR is the exchange of DNA strands, wh...
متن کاملBiochemical Properties of MutL, a DNA Mismatch Repair Endonuclease
DNA mismatch repair (MMR) is one of the most widely conserved DNA repair systems, which repairs mismatched bases generated mainly by the error of DNA polymerases during replication (Friedberg, et al., 2006, Iyer, et al., 2006, Kunkel, et al., 2005, Morita, et al., 2010). MMR increases the replication fidelity by 20 to 400-fold (Schaaper, 1993). Mutations and epigenetic silencing in MMR genes ca...
متن کاملMismatch repair protein MutL becomes limiting during stationary-phase mutation.
Postsynthesis mismatch repair is an important contributor to mutation avoidance and genomic stability in bacteria, yeast, and humans. Regulation of its activity would allow organisms to regulate their ability to evolve. That mismatch repair might be down-regulated in stationary-phase Escherichia coli was suggested by the sequence spectrum of some stationary-phase ("adaptive") mutations and by t...
متن کاملDefects in homologous recombination repair in mismatch-repair-deficient tumour cell lines.
Loss of mismatch repair (MMR) leads to a complex mutator phenotype that appears to drive the development of a subset of colon cancers. Here we show that MMR-deficient tumour cell lines are highly sensitive to the toxic effects of thymidine relative to MMR-proficient lines. This sensitivity was not a direct consequence of MMR deficiency or alterations of DNA precursor metabolism. Instead, MMR-de...
متن کاملMutation detection with MutH, MutL, and MutS mismatch repair proteins.
Escherichia coli methyl-directed mismatch repair is initiated by MutS-, MutL-, and ATP-dependent activation of MutH endonuclease, which cleaves at d(GATC) sites in the vicinity of a mismatch. This reaction provides an efficient method for detection of mismatches in heteroduplexes produced by hybridization of genetically distinct sequences after PCR amplification. Multiple examples of transition...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: DNA Repair
سال: 2009
ISSN: 1568-7864
DOI: 10.1016/j.dnarep.2008.10.011